Table S3: Quantitative real-time RT-PCR assays for 51 transcripts representing highly significant differences between NOA subclassses

OKADA, Hiroyuki, et al. Genome-wide expression of azoospermia testes demonstrates a specific profile and implicates ART3 in genetic susceptibility. PLoS genetics, 2008, 4. Jg., Nr. 2, S. e26.

Publication: https://doi.org/10.1371/journal.pgen.0040026

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The publication Genome-Wide Expression of Azoospermia Testes Demonstrates a Specific Profile and Implicates ART3 in Genetic Susceptibility by Hiroyuki Okada and Atsushi Tajima and Kazuyoshi Shichiri and Atsushi Tanaka and Kenichi Tanaka and Ituro Inoue is published under an open access no derivatives license: : https://creativecommons.org/. Open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Table

GENE	Name	GeneAc.No.	ProbeId(microarray)	Real-timePcr(assay)	Real-timePcr	AssayId	PrimerSequencesForward(5	PrimerSequencesReverse(5
ACAT2	acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)	NM_005891	A_23_P383835	SYBR	TAKARA	HA041816	GCAAGATGAATGCAGGCTCAGA	CAGCAGCTAAGGCACCATTGAA
ART3	ADP-ribosyltransferase 3	NM_001179	A_23_P80918	TaqMan	AB	Hs00154151_m1
BUB1	BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)	NM_004336	A_23_P124417	SYBR	TAKARA	HA039468	GCCATCAAGCCCAAGACTGAA	CCATCAACTGGGTCCCAATGTAG
CDCA2	cell division cycle associated 2	NM_152562	A_23_P385861	SYBR	TAKARA	HA060441	CCTATTCCCGAGCTGCCTGA	CTTCCAGTTTGCCATTTGAACTGA
CDCA5	cell division cycle associated 5	NM_080668	A_23_P104651	SYBR	QIAGEN	Hs_CDCA5_SG_1
CDT1	DNA replication factor	NM_030928	A_23_P37704	SYBR	TAKARA	HA054038	TGGTGGGCAGCTGTTGTACTATC	CTTGTCCAGCTTGACGTAGGTG
CT45-2	cancer/testis antigen CT45-2	NM_152582	A_23_P362076	SYBR	TAKARA	HA060464	ACGCTGTGGCTGGCTACTTC	TAGGGCACCTGGGAGTGATG
CTAG1B	cancer/testis antigen 1B	NM_001327	A_23_P148541	SYBR	TAKARA	HA035019	AGAGCCGCCTGCTTGAGTTC	GCAGCAGTCAGTCGGATAGTCAGTA
DAZL	deleted in azoospermia-like	NM_001351	A_23_P212105	SYBR	TAKARA	HA035049	CACCAGTTCAGGTCATCACTGGATA	CAACTTCAGCTCCTGGATCAACTTC
DDX4	DEAD (Asp-Glu-Ala-Asp) box polypeptide 4	NM_019039	A_23_P256624	SYBR	TAKARA	HA050045	GCTGATCGCATGTTGGATATGG	AGTTGCACTGAACATAAGGGTTTGG
DDX43	DEAD (Asp-Glu-Ala-Asp) box polypeptide 43	NM_018665	A_23_P156445	SYBR	TAKARA	HA049687	AAGCAGACAAGATGTTGGACATGG	GTGCGAGGCGATGAACTGAA
DMRTB1	DMRT-like family B with proline-rich C-terminal; 1	NM_033067	A_23_P417821	SYBR	TAKARA	HA055770	CACTGACCGTCCTGTTTGATACTGA	CCTAGTCGGACTGCTCCTGAGA
DMRTC2	DMRT-like family C2	NM_033052	A_23_P300245	TaqMan	AB	Hs00540564_m1
DSCR8	Down syndrome critical region gene 8	NM_032589	A_23_P6252	TaqMan	AB	Hs00741223_m1
EGR4	early growth response 4	NM_001965	A_23_P380318	SYBR	TAKARA	HA035926	CCATGCTCCACCTTAGCGAGTT	GCTGTTCAAAGCCCAGCTCAA
FGFR3	fibroblast growth factor receptor 3 (achondroplasia; thanatophoric dwarfism)	NM_000142	A_23_P500501	SYBR	TAKARA	HA031790	CACCCTACGTTACCGTGCTCAA	AGCCACGCAGAGTGATGAGAA
FMR1NB	fragile X mental retardation 1 neighbor	NM_152578	A_23_P396112	SYBR	TAKARA	HA060460	TCTAAAGATGCGGGTCAGCAAAC	CAGGATATGTCCATTTGCAAGCAC
GAJ	GAJ protein	NM_032117	A_23_P133123	SYBR	TAKARA	HA054663	GTTGATGATGGTATGGTTGACTGTG	CCCTCAGACAACTGAGATTCCAGA
HIST1H2AA	histone 1; H2aa	NM_170745	A_23_P355439	SYBR	TAKARA	HA061653	AGCTAGCGATCCGCAATGATG	TGCCTGAATGTTAGGCAGGACTC
HIST1H2BA	histone 1; H2ba	NM_170610	A_23_P323821	TaqMan	AB	Hs00601111_s1
HNRNPG-T	testes-specific heterogenous nuclear ribonucleoprotein G-T	NM_014469	A_23_P61967	SYBR	TAKARA	HA045528	CGAAGCCGAGTTTGGCAAGTA	GGACTTGCCGTTCATGTCTCTG
HORMAD1	HORMA domain containing protein	NM_032132	A_23_P327530	SYBR	TAKARA	HA054688	CCCAGAATGCGCTTATGGAAC	ACTAACTGTGTAGATCCTGGGCAAT
INHBB	inhibin; beta B (activin AB beta polypeptide)	NM_002193	A_23_P153964	SYBR	TAKARA	HA036226	AAATCATCAGCTTCGCCGAGAC	GACGTAGGGCAGGAGTTTCAGGTA
LOC112714	similar to alpha tubulin	NM_207312	A_23_P56736	SYBR	TAKARA	HA026753	CTGGAAGTAGCGTTGGGCTGA	CATTTGACCATCGGGCTGAA
LOC158812	hypothetical LOC158812	XM_088679	A_23_P255457	SYBR	QIAGEN	Hs_LOC158812_SG_1
LOC255313	hypothetical protein LOC255313	NM_173571	A_23_P305074	SYBR	TAKARA	HA062398	CCCATCTTAGGGTGTCGTCTGAG	GACCACGGTGAAGACGGTGA
LOC92196	similar to death-associated protein	ENST00000309950	A_23_P165598	SYBR	QIAGEN	Hs_LOC92196_SG_1
MAGEA2	melanoma antigen; family A; 2	NM_005361	A_23_P148255	SYBR	QIAGEN	Hs_MAGEA2_SG_1
MGC40042	similar to mouse meiosis defective 1 gene	AK093341	A_23_P211561	TaqMan	AB	Hs00415170_m1
MTL5	metallothionein-like 5; testis-specific (tesmin)	NM_004923	A_23_P161507	SYBR	TAKARA	HA040397	CATGGCCTGAGCATTGTTTGTC	GCAGCCTCCAGAGCAACATTC
NYD-SP20	testes development-related NYD-SP20	NM_032598	A_23_P101084	SYBR	TAKARA	HA055218	TGGCGTGAACATGCACAGAA	TTCGAATAATATGGGCCAGGTGTAA
OTEX	paired-like homeobox protein OTEX	NM_139282	A_23_P85083	SYBR	TAKARA	HA058753	CCGACACACTCAATACCCTGATG	CGCACTTTGTCTTCAGTCACACC
PAGE1	P antigen family; member 1 (prostate associated)	NM_003785	A_23_P171104	TaqMan	AB	Hs00269455_m1
PAGE5	P antigen family; member 5	NM_130467	A_23_P22744	TaqMan	AB	Hs00373098_m1
PEPP-2	PEPP subfamily gene 2	AL590524	A_23_P73677	SYBR	TAKARA	HA055095	TCCATCCACACCACTCAGGGTA	GGGCTGAGCAAGCTGGTCATA
PIK3R3	phosphoinositide-3-kinase; regulatory subunit; polypeptide 3 (p55; gamma)	NM_003629	A_23_P22970	SYBR	QIAGEN	Hs_PIK3R3_SG_1
RAD54L	RAD54-like (S. cerevisiae)	NM_003579	A_23_P74115	SYBR	TAKARA	HA038276	CGAAGCCGTAGCAGTGACAAAG	ATGGACATCGTGCCATCCAG
SPAG5	sperm associated antigen 5	NM_006461	A_23_P89509	TaqMan	AB	Hs00197708_m1
SSX6	synovial sarcoma; X breakpoint 6	NM_173357	A_23_P33881	SYBR	QIAGEN	Hs_SSX6_SG_1
STAG3	stromal antigen 3	NM_012447	A_23_P145657	SYBR	TAKARA	HA044533	TCCTAGCCGGGTTCTGCAAG	CAGCAGGATTCGGGAACAATG
SYCP3	synaptonemal complex protein 3	NM_153694	A_23_P76374	SYBR	TAKARA	HA061362	TTCTGACTTTGTTTCAGCAGTGG	CAATCTCTGGCTCTGAACAATTCTA
TEX14	testis expressed sequence 14	NM_031272	A_23_P54996	SYBR	TAKARA	HA054180	AAGCGACTAGGTTGGTCCGAATC	CTGGAACTGCTGCCCTGACA
TKTL1	transketolase-like 1	NM_012253	A_23_P259901	SYBR	TAKARA	HA044282	CTCGCTGAGAGCAACATCAACATTA	GAGACGGCATCAGTTGGGTAGAA
TSPY1	testis specific protein; Y-linked 1	NM_003308	A_23_P22800	SYBR	TAKARA	HA037903	CTGGCTTCTGGGCCAATGTTA	CATGATCTTGCAGAGATGAACAGGA
TSPY2	testis specific protein; Y-linked 2	NM_022573	A_23_P34200	SYBR	TAKARA	HA052164	GCCTTCAGGATGGAGGCTGT	AAAGCCGCCTGCTCACTTTC
TSPY2	testis specific protein; Y-linked 2	NM_022573	A_23_P251391	SYBR	TAKARA	HA052164	GCCTTCAGGATGGAGGCTGT	AAAGCCGCCTGCTCACTTTC
VCX	variable charge; X-linked	NM_013452	A_23_P252572	SYBR	TAKARA	HA044908	AGATGAGTCCAAAGCCGAGAG	TTGGCCACCTTGGTAGTCTTC
VCY	variable charge; Y-linked	NM_004679	A_23_P11390	SYBR	TAKARA	HA040018	GACCGTTGCGAGACGTTGAG	CTCGGCCACCTTGGTAGTCTTC
XAGE1	X antigen family; member 1	NM_133431	A_23_P383310	SYBR	TAKARA	HA057720	TCCAACTGGGAGTTGAAGTGTGAG	AGTTATGGCCGTGACGAGACAAG
XAGE3	X antigen family; member 3	NM_130776	A_23_P114343	SYBR	QIAGEN	Hs_GAGED4_SG_1
XAGE5	X antigen family; member 5	NM_130775	A_23_P301497	TaqMan	AB	Hs00793258_g1