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The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKA

T Wang, S Young, H Krenz, F Tüttelmann, A Röpke, C Krallmann, S Kliesch, XH Zeng, C Brenker, T Strünker., 18.09.2020

WANG, Tao, et al. The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKA. Journal of Biological Chemistry, 2020, 295. Jg., Nr. 38, S. 13181-13193.

Publication: https://doi.org/10.1074/jbc.RA120.013218

Disclaimer

The publication The Ca2+ channel CatSper is not activated by cAMP/PKA signaling but directly affected by chemicals used to probe the action of cAMP and PKA by T Wang, S Young, H Krenz, F Tüttelmann, A Röpke, C Krallmann, S Kliesch, XH Zeng, C Brenker, T Strünker. is not published under an open access license. Therefore, only content curated by the MFGA team can be shown. For abstract, images and tables please follow the link to the original publication.

Curation by the MFGA team
Relevant data sets presented in the publication have been identified. If possible, annotations (title, general information, conditions, processed tissue types and processed cell types) have been added based on information from the publication.

Data set 1: CatSper is not activated by intracellular cAMP or cAMP/PKA signaling

Proteome: Functional Study

Species

Species
Human
Sea Urchin

Conditions

Human phenotype ontology Participants Comment
HP:control

Cell Types

Cell ontology Maturity Description Species Replicates Cells per replicate
CL_0000019: sperm sample sizes (50 versus 16)

Data set 2: Membrane-permeable cAMP analogs activate CatSper only from the outside

Proteome: Functional Study

Species

Species
Human

Conditions

Human phenotype ontology Participants Comment
HP:control 3
HP:other: Other 3 Sperm lacking functional CatSper channels (CATSPER2−/−).

Cell Types

Cell ontology Maturity Description Species Replicates Cells per replicate
CL_0000019: sperm

Data set 3: Human CatSper is activated by cyclic nucleotides via a so-far-unknown binding site

Proteome: Functional Study

Data set 4: The action of PKA inhibitors on CatSper does not rest on inhibition of PKA

Proteome: Functional Study

Species

Species
Human

Conditions

Human phenotype ontology Participants Comment
HP:control

Cell Types

Cell ontology Maturity Description Species Replicates Cells per replicate
CL_0000019: sperm