Table S2: Whole-mount Immunofluorescence for identification of ex vivo hPGCs
CHEN, Di, et al. Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors. Cell reports, 2019, 29. Jg., Nr. 13, S. 4568-4582. e5.
Publication: https://doi.org/10.1016/j.celrep.2019.11.083
Description
We discovered N/S/T triple-positive putative ex vivo hPGCs at day 12 post-fertilization in 2/26 embryos.
Disclaimer
The publication Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors by Di Chen, Na Sun, Lei Hou, Rachel Kim, Jared Faith, Marianna Aslanyan, Yu Tao, Yi Zheng, Jianping Fu, Wanlu Li, Manolis Kellis, Amander Clark is published under an open access no derivatives license: : https://creativecommons.org/licenses/by-nc-nd/4.0/. Granted rights: share — copy and redistribute the material in any medium or format. No alterations allowed. Thus, tables can not be shown in MFGA format.
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Table
| DayImaged | AttachmentCulture | TotalAttachmentsNanog+ | TotalAttachments_sox17+ | TotalImaged | TotalAttachments_tfap2+ | TotalAttachments_nanog+_sox17+ | TotalAttachments_nanog+_sox17+_tfap2c+ |
|---|---|---|---|---|---|---|---|
| D10 | D6 + 4 | 8 (62%) | 9 (69%) | 13 | 13 | 0 | 0 |
| D10 | D7 + 3 | 2 (100%) | 1 (50%) | 2 | 2 | 0 | 0 |
| D11 | D6 + 5 | 3 (50%) | 0 (0%) | 6 | 6 | 0 | 0 |
| D11 | D7 + 4 | 2 (33%) | 3 (50%) | 6 | 6 | 0 | 0 |
| D11 | D8 + 3 | 0 (0%) | 0 (0%) | 1 | 1 | 0 | 0 |
| D12 | D6 + 6 | 5 (29%) | 4 (24%) | 17 | 17 | 1 (5.9%) | 1 (5.9%) |
| D12 | D7 + 5 | 5 (56%) | 3 (33%) | 9 | 9 | 1 (11%) | 1 (11%) |
| Total | 25 (46%) | 20 (37%) | 54 | 54 (100%) | 2 (3.7%) | 2 (3.7%) |