Table S1: List of primary antibodies and respective isotype controls used in this study
GOMES FERNANDES, Maria, et al. Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis. MHR: Basic science of reproductive medicine, 2018, 24. Jg., Nr. 5, S. 233-243.
Publication: https://doi.org/10.1093/molehr/gay011
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The publication Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis by Maria GomesFernandes, Monika Bialecka, Daniela C F Salvatori, Susana M Chuva de Sousa Lopes is published under an open access license: https://creativecommons.org/licenses/by-nc/4.0/. Permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Curation by the MFGA team
Pie chart of selected column
Table
| PrimaryAntibodies | Isotype | Manufacturer | Cat.No. | Dilution | IsotypeControl | Manufacturer | Cat.No. |
|---|---|---|---|---|---|---|---|
| Goat anti-POU5F1 | IgG | Santa Cruz Biotechnology | sc-8628 | 04:20:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Mouse anti-POU5F1 | IgG2b | Santa Cruz Biotechnology | sc-5279 | 04:20:00 AM | Mouse IgG2b | BD Biosciences | 555740 |
| Rat anti-PRDM1 | IgG2b | eBioscience | 14-5963-82 | 01:50:00 AM | Mouse IgG2b | BD Biosciences | 555740 |
| Rat anti-PRDM1 | IgG2a | Santa Cruz Biotechnology | sc-47732 | 01:50:00 AM | Rat IgG2a | R&D Systems | MAB006 |
| Goat anti-SOX17 | IgG | R&D Systems | AF1924 | 04:20:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Goat anti-ALPL | IgG | R&D Systems | AF2910-SP | 04:20:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Rabbit anti-KIT | IgG | DAKO | A50229 | 04:20:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Rabbit anti-PECAM1 | IgG | Santa Cruz Biotechnology | sc-1506-R | 04:20:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-ITGB1 | IgG | Chemicon | ab1952 | 02:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Goat anti-NANOG | IgG | R&D Systems | AF1997 | 04:20:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Rabbit anti-IFITM3 | IgG | Abcam | ab15592 | 04:20:00 AM | Rabbit IgG | X0903 | DAKO |
| Mouse anti-CD38-AF700 | IgG1 | BioLegend | 303523 | 04:20:00 AM | Mouse IgG1 | BioLegend | 400143 |
| Goat anti-T | IgG | R&D Systems | AF2085 | 02:40:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Rabbit anti-GATA6 | IgG | Santa Cruz Biotechnology | ab9055 | 04:20:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-DPPA3 | IgG | Abcam | ab19878 | 02:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-SOX2 | IgG | Merck | ab5603 | 04:20:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-TFAP2C | IgG | Santa Cruz Biotechnology | sc-8977 | 02:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Mouse anti-CXCR4 | IgG2b | R&D Systems | MAB173 | 04:20:00 AM | Mouse IgG2a | DAKO | X094301 |
| Mouse IgM anti-TRA-1-81 | IgM k | Merck | MAB4381 | 01:50:00 AM | Mouse IgM | BD Biosciences | 555581 |
| Mouse IgM anti-SSEA1 | IgM k | Santa Cruz Biotechnology | sc-21702 | 01:50:00 AM | Mouse IgM | BD Biosciences | 555581 |
| Mouse anti-SSEA4 | IgG3 | Santa Cruz Biotechnology | sc-59368 | 01:50:00 AM | Mouse IgG3 | Miltenyi Biotec | 130-095-623 |
| Mouse anti-SALL4 | IgG1 | Santa Cruz Biotechnology | sc-101147 | 02:40:00 AM | Mouse IgG1 | BioLegend | 400143 |
| Goat anti-ITGA6 | IgG | Santa Cruz Biotechnology | sc-6597 | 02:40:00 AM | Goat IgG | SantaCruzBiotechnology | sc-2028 |
| Rabbit anti-H3K27me3 | IgG | Merck | 07-449 | 02:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Mouse anti-5mC | IgG1 | Abcam | ab10805-50 | 04:20:00 AM | Mouse IgG1 | BioLegend | 400143 |
| Rabbit anti-CDH1 | IgG | Cell Signaling Technology | 3195S | 02:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Goat anti-CDH2 | IgG | Santa Cruz Biotechnology | sc-1502 | 02:40:00 AM | Goat IgG | Santa Cruz Biotechnology | sc-2028 |
| Rabbit anti-CDH5 | IgG | Cell Signaling Technology | 2158S | 01:50:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-TUBB3 | IgG | Sigma-Aldrich | T2200 | 04:20:00 AM | Rabbit IgG | X0903 | DAKO |
| Rabbit anti-PIWIL4 | IgG | Abcam | ab87939 | 07:40:00 AM | Rabbit IgG | X0903 | DAKO |
| Mouse anti-EPCAM-VioBlue | IgG1 | Miltenyi Biotec | 130-098-092 | 01:50:00 AM | Mouse IgG1 | Miltenyi Biotec | 130-098-845 |
| Rat anti-PDPN-PE | IgG2a | Thermo Fischer Scientific | 12-9381-42 | 12:40:00 PM | Rat IgG2a | Thermo Fischer Scientific | 12-4321-80 |
| Rabbit anti-5hmC | IgG | Active Motif | 39792 | 09:20:00 AM | Rabbit IgG | X0903 | DAKO |