Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis
Mei Wang, Xixi Liu, Gang Chang, Yidong Chen, Geng An, Liying Yan, Shuai Gao, Yanwen Xu, Yueli Cui, Ji Dong, Yuhan Chen, Xiaoying Fan, Yuqiong Hu, Ke Song, Xiaohui Zhu, Yun Gao, Zhaokai Yao, Shuhui Bian,…Jie Qiao, 30.08.2018
WANG, Mei, et al. Single-cell RNA sequencing analysis reveals sequential cell fate transition during human spermatogenesis. Cell Stem Cell, 2018, 23. Jg., Nr. 4, S. 599-614. e4.
Publication: https://doi.org/10.1016/j.stem.2018.08.007
Disclaimer
The publication Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis by Mei Wang, Xixi Liu, Gang Chang, Yidong Chen, Geng An, Liying Yan, Shuai Gao, Yanwen Xu, Yueli Cui, Ji Dong, Yuhan Chen, Xiaoying Fan, Yuqiong Hu, Ke Song, Xiaohui Zhu, Yun Gao, Zhaokai Yao, Shuhui Bian,…Jie Qiao is not published under an open access license.
Therefore, only content curated by the MFGA team can be shown.
For abstract, images and tables please follow the link to the original publication.
Curation by the MFGA team
Relevant data sets presented in the publication have been identified. If possible, annotations (title, general information,
conditions, processed tissue types and processed cell types) have been added based on information from the publication.
Data set 1: Global Transcriptional Profiling of Adult Human Testicular Cells and Cell Type Identification
Transcriptome: Single-cell RNA-Sequencing
Conditions
| Human phenotype ontology |
Participants |
Comment |
| HP:0011961: Non-obstructive azoospermia |
1.0 |
174 testicular cells |
| HP:control |
|
2,854 individual testicular cells from donors with normal spermatogenesis (donors with normal fertility or obstructive azoospermia [OA]) |
Tissue Types
| BRENDA tissue ontology |
Maturity |
Description |
Species |
Replicates |
| BTO_0001363: testis |
Adult |
|
Human |
|
Cell Types
| Cell ontology |
Maturity |
Description |
Species |
Replicates |
Cells per replicate |
| CL_0000586: germ cell |
Adult |
|
Human |
|
|
| PLANA_0000219: spermatogonial stem cell |
Adult |
SSC; Gh4+, nanos+ cells residing at the testis periphery that self-renew and give rise to differentiating daughters that will ultimately produce haploid sperm. |
Human |
|
|
| CL_0000020: spermatogonium |
Adult |
Differentiating and differentiated spermatogonia |
Human |
|
|
| CL_0000216: Sertoli cell |
Adult |
|
Human |
|
|
| CL_0002371: somatic cell |
Adult |
Testicular somatic cells |
Human |
|
|
| CL_0000018: spermatid |
Adult |
|
Human |
|
|
Data set 2: scRNA-Seq Data of Human Spermatogenesis as a Reference for the Diagnosis of Male-Reproduction-Related Disorders and a Platform for the Dissection of the Spermatogenesis Conservation between Human and Mouse
Transcriptome: Other
Conditions
| Human phenotype ontology |
Participants |
Comment |
| HP:0011961: Non-obstructive azoospermia |
1.0 |
|
| HP:control |
|
Normal Spermatogenesis |
Tissue Types
| BRENDA tissue ontology |
Maturity |
Description |
Species |
Replicates |
| BTO_0001363: testis |
Adult |
NOA, 174 cells |
Human |
1 |
Cell Types
| Cell ontology |
Maturity |
Description |
Species |
Replicates |
Cells per replicate |
| CL_0000178: Leydig cell |
Adult |
NOA |
Human |
|
|
| CL_0002481: peritubular myoid cell |
Adult |
NOA- peritubular myoid cells |
Human |
|
|
