Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility
Frédéric Chalmel, Aurélie Lardenois, Bertrand Evrard, Romain Mathieu, Caroline Feig, Philippe Demougin, Alexandre Gattiker, Wolfgang Schulze, Bernard Jégou, Christiane Kirchhoff Michael Primig, 27.08.2012
CHALMEL, Frédéric, et al. Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility. Human reproduction, 2012, 27. Jg., Nr. 11, S. 3233-3248.
Publication: https://doi.org/10.1093/humrep/des301
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The publication Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility by Frédéric Chalmel, Aurélie Lardenois, Bertrand Evrard, Romain Mathieu, Caroline Feig, Philippe Demougin, Alexandre Gattiker, Wolfgang Schulze, Bernard Jégou, Christiane Kirchhoff Michael Primig is not published under an open access license. Therefore, only content curated by the MFGA team can be shown. For abstract, images and tables please follow the link to the original publication.
Curation by the MFGA team Relevant data sets presented in the publication have been identified. If possible, annotations (title, general information, conditions, processed tissue types and processed cell types) have been added based on information from the publication.
Data set 1: Transcriptional Profiling and Spatio-temporal association between gene expression and gene function in human testis
Transcriptome: Bulk RNA-Sequencing
Species
| Species |
|---|
| Human |
Conditions
| Human phenotype ontology | Participants | Comment |
|---|---|---|
| HP:0000789: Infertility | 30 | Samples from infertile patients whose seminiferous tubules were either empty (mJS 1, n=1; De Kretser and Holstein, 1976) or contained almost exclusively Sertoli cells (mJS 2, n=7) or Sertoli cells but rarely spermatogonia (mJS 3, n=3). These cases were compared with samples containing spermatocytes but no spermatids (mJS 5, n=8), early but no late spermatids (mJS 7, n=4) and many early and elongated but only few mature spermatids (mJS 8, n=7) |
| HP:control | 8 | vasectomized patients showing normal spermatogenesis as controls (mJS n=8) in addition, the patient samples were compared with previously published data obtained with highly enriched spermatocytes, spermatids, seminiferous tubules and total-testis controls from fertile individuals |
| HP:other: Other | 9 | Prepubertal children whose testes contain typical (Ad+, n=5 replicates) or very low levels (Ad2, n=4) of Adark spermatogonial cells |
Tissue Types
| BRENDA tissue ontology | Maturity | Description | Species | Replicates |
|---|---|---|---|---|
| BTO_0001363: testis | Infant | Prepubertal | Human | |
| BTO_0001363: testis | Adult | Human |
Cell Types
| Cell ontology | Maturity | Description | Species | Replicates | Cells per replicate |
|---|---|---|---|---|---|
| CL_0000017: spermatocyte | Adult | Human | |||
| CL_0000018: spermatid | Adult | Human | |||
| CL_0000216: Sertoli cell | Adult | Human | |||
| CL_0000020: spermatogonium | Adult | Human | |||
| CL_0000178: Leydig cell | Adult | Human | |||
| CL_0002481: peritubular myoid cell | Adult | Human | |||
| CL_0000020: spermatogonium | Infant | prepubertal | Human |